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The K m value for CDNB was obtained by reacting 0.5 g of purified e-GSTs of mammals with variable amounts of CDNB (from 0.05 to 2 mM) in the presence of 1 mM GSH, in 0.1 M potassium phosphate buffer, pH 6.5 (25 C)

Proper storage and careful handling help preserve peptide integrity and support consistent experimental outcomes

If SOD is either functioning poorly or is present in inadequate quantities, rampant oxidative stress driven by unabated superoxide molecules can damage tissue and contribute to disease

Some researchers also suggest glutathione can directly bind with the active site of tyrosinase (which contains copper), effectively inactivating the enzyme and further halting melanin synthesis