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At the end point, cells were rapidly washed with ice-cold PBS and incubated at 80 C for 30 min with extraction solvent at a ratio of 1 ml per 1 10 6 cells

Once bound, these toxins become water-soluble and can be transported out of cells

10.1021/acs.chemrev.7b00205 [DOI] [PMC free article] [PubMed] [Google Scholar] Forman HJ, Zhang H (2021) Targeting oxidative stress in disease: promise and limitations of antioxidant therapy

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Alvarez FJ, Lafuente H, Rey-Santano MC, Mielgo VE, Gastiasoro E, Rueda M, et al