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Acknowledgements This work was supported by grants from The Regional Innovation and Development Joint Fund of National Natural Science Foundation of China (No

Sample type considerations Different biological matrices have different detection characteristics: Urine: Most common testing matrix, metabolites accumulate Blood/plasma: Shorter detection windows for most peptides Dried blood spots: Emerging method with good sensitivity Hair: Not commonly used for peptides but theoretically possible Most anti-doping testing uses urine

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[1] [2] These mechanisms typically lead to reduced caloric intake and subsequent weight loss in most patients