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Ou Q, Wang L, Wang K, Shao P

Features of Protein A/G Magnetic Beads: High capacity nearly four times higher binding capacity than typical magnetic beads from other suppliers, allowing the use of smaller amounts per experiment Low non-specific binding stable, pre-blocked beads provide clean purification products (e.g., antigen eluted in IP with antibody is devoid of contaminating proteins from complex IP matrix) Flexibility convenience of IgG binding domains of both Protein A and Protein G on one bead Compatibility beads are compatible with manual and automated applications (e.g., Thermo Scientific KingFisher Instruments) Assay consistency magnetic beads eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes These magnetic beads are coated with genetically engineered Pierce Protein A/G, a recombinant fusion protein which combines the IgG binding domains of both Protein A and Protein G

These changes did not occur in all lymphocytes but were only partially visible ( Figure 7D )

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