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PC cells were harvested after trypsinization, followed by centrifugation and then fixed at 4 C overnight in Karnovskys fixative (2% paraformaldehyde and 5% glutaraldehyde in 0.1 M cacodylate, pH 7.4)

Amino Acids, 53 (4), 575585

We showed that the activity of cysteine proteases (falcipain2 and 3, and dipeptidyl aminopeptidase 1) 43 involved in hemoglobin digestion were significantly decreased in 24 hpi artemisinin resistant parasites compared to sensitive (Fig 2C), while proteomics analysis showed no difference in the abundance of these proteins (Fig 2D) 30

That small percentage of preservative prevents bacterial growth inside the vial after you puncture the rubber stopper

This 10-15 second exam is an important way to find problems