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After protein expression, E.coli cells are harvested by centrifugation, superatant is removed and cell pellet is resuspended in lysis buffer of choice

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Acknowledgments We acknowledge the support from the Department of Developmental Cell Biology, Cell Biology Division, Key Laboratory of Cell Biology, Ministry of Public Health, Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University

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He was 30 years older than Khanyi