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Additionally, if you're taking other medications that affect fluid balancesuch as diuretics, ACE inhibitors, NSAIDs, or SGLT2 inhibitorsinform your healthcare provider about any urination changes, as drug interactions may compound dehydration risk

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After fixation, the cells were washed with PBS and permeabilized with 0.1% Triton X-100 (Daejung 8566-4405) in PBS for 10 min at room temperature