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Because inhomogeneous cellular distribution of reacted and unreacted probes may bias the ratiometric calculations, especially at the subcellular resolution, we opted for RT instead of RT-NH 2 to achieve high-quality quantitative results in living cells

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Subsequently summarized in J

Minor differences in the appearance of the lyophilized peptide (particularly color) between production lots are normal and generally reflect variations in copper content and freeze-drying conditions rather than differences in peptide identity or analytical purity

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