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Bhatta, S

Generation of YPEL5 -mutant cells HEK293T cells were transfected with either pN106/YPEL5-FLAG-WT, pN106/YPEL5-FLAG-T36A, pN106/YPEL5-FLAG-R41A or pN106/YPEL5-FLAG-L62A, along with pMD2.G (Addgene, 12259) and psPAX2 (Addgene, 12260), using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus

Reconstitute: Add 3.0 mL bacteriostatic water ~ 1.67 mg/mL concentration

Time frame and selection criteria: The focus was primarily on articles published in the last two decades, with particular attention to comprehensive reviews, landmark studies, and significant clinical trials

There is currently no established evidence that GLP-1 receptor agonists directly treat or improve Hashimoto's disease or its underlying autoimmune pathology

C-Met is expressed by highly autoreactive encephalitogenic CD8+ cells