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Optimized the lipid-to-peptide ratio to prevent leakage in the presence of surfactants found in shampoos

For the reporter assay, cells were transfected with a Gaussia luciferase (GLUC) plasmid containing either the human SIRT1 promoter (HRPM35661-PG02, Genecopoeia, USA) or the mouse HK2 promoter (MPRM42494-PG02, Genecopoeia) sequence, along with a Renilla luciferase plasmid

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