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cDNA was synthesized and then quantitative PCR (qPCR) amplification was conducted with the C1000 Thermal Cycler (Bio-Rad, catalog CFX384) using iQ SYBR Green Supermix (Bio-Rad)

Mol Cell Endocrinol (2020) 500:110628

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For the expression of the MAX2ASK1 proteins, the amplified virus was used to infect Sf9 cell cultures at a density of 2 10 6 cells/mL

Taken together, these findings suggest an immunometabolic checkpoint that could be adopted as a pre-symptomatic biomarker and may also be therapeutically targeted alone or in combination with other immunotherapies for disease modification in AD

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