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Nissl staining Mouse brain tissue was collected, fixed in 4% paraformaldehyde for 48 h, and embedded in paraffin

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in-vitro study found that berberine protected NSC34 cells from H 2 O 2 -induced cytotoxicity by reducing ROS production, restoring glutathione, and superoxide dismutase activity, and activating the production of antioxidant proteins nuclear factor erythroid 2-related factor-2 (Nrf2) and HO-1 ( Studies have found that berberine has an antidepressant effect (Figure 1)

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P -values for the group effect were extracted from the model coefficients, and significance was determined using false discovery rate correction (BenjaminiHochberg method), with adjusted P -value log 2 (1.25)