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GSTs bind the ROS substrate at its hydrophobic alpha-helical C-terminal domain, as well as reduced glutathione to its glutathione-binding site on the N-terminal domain

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Subsequently, each system underwent independent simulations lasting 500 ns each (totaling 1.5 s per system) at 298 K, employing the Langevin thermostat with a time step of 0.2 fs (Supplementary Fig

Repeated freeze-thaw cycles degrade peptide integrity and can introduce variability into your experimental results always prepare only what you need for immediate use

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