However, one of the most important limitations is heterogeneity of the study populations, including disease type, tolerance to treatment, dose of DHA and EPA, timing and duration of DHA and EPA supply, EPA to DHA ratio, initial EPA and DHA status, intake of other nutrients such as vitamins, clinical status, and concomitant use with medications
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Trial populations have included a wide age range
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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min
