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The osteoclasts were TRAP-positive cells with more than three nuclei, and the area and number of these osteoclasts were quantified using the ImageJ software (NIH, Bethesda, MD, USA)

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Whereas, sample B showed a spherical shape of the particles inclined to hexagonal shape with a diameter ranges from 20 to 50 nm, and heterogeneous distribution (Fig

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Biochem Biophys Res Commun (2018) 495(1):103440