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In brief, hFSP1 purified from Ni-Chelating resin was firstly denatured by heating at 85 C for 10 min, followed by centrifugation at 13,000 g for 20 min

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Hypertrophic adipocytes can promote the infiltration of macrophages into adipose tissue, thereby creating a local microenvironment characterized by a pro-inflammatory state, manifested by increased production of various cytokines, including tumor necrosis factor- (TNF-), interleukin-6 (IL-6), and chemokines