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Two-photon excitation used a Newport-Spectra Physics Mai Tai Ti:Sapphire Laser pulsing at 80 MHz, and a Zeiss LSM780 (Oberkochen, Germany) microscope with a 20 water immersion objective (NA 1.0)
[CrossRef] [PubMed] [Google Scholar] Neuroprotective effects of metformin through the modulation of neuroinflammation and oxidative stress
Footnotes Conflict-of-interest statement: All the authors declare no conflicts of interest
As a control, cells were transfected with non-targeting siRNA (siCtr)
As further consideration of translation of this therapeutic to the clinic, cochlear synaptopathy is implicated as a potential contributor to human tinnitus, although the published evidence is emerging and, so far, is largely indirect 11,60
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