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[DOI] [PMC free article] [PubMed] [Google Scholar] 90.Gu F., Chauhan V., Kaur K., Brown W.T., LaFauci G., Wegiel J., Chauhan A

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Ophthalmology 115 , 14471449 (2008)

The eluted CCL1-CCR8-Gi complex was mixed with scFv16 on ice for 1 h and further purified by size-exclusion chromatography on a Superdex 200 3.2/300 column in 20 mM Hepes, pH 7.5, 150 mM NaCl and 0.00075% (w/v) LMNG, 0.00025% glyco-diosgenin (GDN), 0.0002% (w/v) CHS (Anatrace)