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What they did: A review article by Thomas Lutz (University of Zurich), one of the main authors in amylin biology
The slides were counterstained with 4,6-diamidino-2-phenylindole (DAPI) and observed under a fluorescence microscope
400 l of the reagent mixture (30 ml glacial acetic acid, 20 ml phosphoric acid and 1.25 g ninhydrin) was mixed with 200 l supernatant and heated in sealed test tubes at 100 C for 1 h
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