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The most classical way of interaction between the gut microbiota and the host is via the metabolites the bacteria produce

Among tryptophan metabolites, serotonin (5-HT) is known to aggravate fibrosis by enhancing inflammation, neutrophil infiltration, oxidative stress, and upregulating TGF-1 and type I collagen [113]

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Maintenance isnt an afterthought

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If cysteine modification is a component required for either HLA-DRB1 binding or TCR recognition, then omission may alter results and potentially fail to highlight immunogenicity risk Presentation of peptides from biotherapeutic proteins is a necessary, but not sufficient, step for developing an ADA response