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Formation of presynaptic terminals at predefined sites along axons

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If you are not going to use the full reconstituted vial within 28 days, consider whether your protocol requires you to reconstitute the full vial at once, or whether you can keep the lyophilised remainder sealed and reconstitute in smaller portions as needed

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Room temperature vs refrigerated after puncture: USP guidance describes the 28-day window under controlled room temperature conditions