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Adv Chronic Kidney Dis (2015) 22(3):20410

Free Radic Biol Med 1992, 12:337345

For cell assays, cells were cultured on coverslips in 24-well plates overnight, fixed with 4% PFA, permeabilized with 0.15% Triton X-100, and blocked with 10% BSA

Phenoxyl radicals of Amplex Red disproportionate to form Amplex Red and resorufin

These physiological changes occur regardless of exercise frequency, making caloric deficit achievable through dietary adjustment alone in many patients, though long-term outcomes may be optimized with some movement