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The activation phase, the second step in the canonical pathway, is driven by diverse stimuli such as K + efflux, ROS overproduction, lysosomal rupture, mitochondrial DNA (mtDNA) leakage, and organelle dysfunction (e.g., mitochondria, Golgi apparatus, endoplasmic reticulum) ( + efflux and thus canonical NLRP3 assembly, has also been suggested ( + efflux, which has been exclusively characterized in primary human and porcine monocytes

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