To test the effect of Sp in paraquat-induced oxidative stress model, seven days old wild type flies, constitutively expressing mitochondrial (mt)-roGFP, were exposed to 10 mM paraquat for 24 h and then co-treated with 2.5 mM Sp for 24 h, prior to roGFP fluorescence estimation
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doi: 10.3389/fpls.2024.1373801 Received 20 January 2024 Accepted 23 February 2024 Published 12 March 2024 Volume 15 - 2024 Edited by Ahmed Abdelkhalek, City of Scientific Research and Technological Applications, Egypt Reviewed by Nuria Montes, Hospital Universitario de La Princesa, Spain Updates Copyright 2024 Kozie, Otulak-Kozie and Rusin
2) Resuspend the cells in 10 mL of Equilibration buffer (LE buffer) with appropriate amount of PMSF (Final working concentration can be 1mM) and add lysozyme (Final working concentration is 0.2-0.4 mg/mL), or other protease inhibitors cocktail added
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