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[DOI] [PubMed] [Google Scholar] 7.Saika S

Nature 2015;520:18691

Animals and Grouping Twenty male Wistar rats were obtained from the Central Animal House, College of Medicine, University of Ibadan

By layering dissociated spinal cord cells over a four-step discontinuous gradient of 4.5%, 6%, 7.5% and 10.5% (w/v) iodixanol (OptiPrep initially diluted to 30% iodixanol with 0.15 M NaCl, 10 mM MOPS, pH 7.4 and then further diluted with Hanks Balanced Salt Solution) and centrifuging at 1900 rpm for 15 min., debris remained at the top interface, neurons banded at the three lower interfaces and inflammatory cells and glial cells pelleted [22,23]

Food and Drug Administration (FDA) do not monitor the safety or effectiveness of supplements, so it is important to choose a reputable brand

Psychiatry , 2013 , 52 (3), 231-240