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Upadhyay et al ., showed that ibuprofen induced proteasome dysfunction, mitochondrial-mediated apoptosis, and cell death in A549 and/or Cos-7 cells 38

Anales de Dermatologa , 28 (4), 438.

Tissues were dissected, post-fixed for 3 h in 4% PFA at room temperature 1820 2 C cryoprotected in 30% (w/v) sucrose in Tris-Azide buffer (40 mM Tris, 10 mM phosphate, 0.7% (w/v) NaCl, 0.05% (w/v) NaN 3 ) at 4 C, then embedded in optimal cutting temperature (OCT) compound, rapidly frozen in isopentane on dry ice, and stored at 80 C until use

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[DOI] [PubMed] [Google Scholar] Falco AM, McDonald CG, Bachus SE, Smith RF (2014) Developmental alterations in locomotor and anxiety-like behavior as a function of D1 and D2 mRNA expression

This ability is attributed to several components, including carotenoids, polyphenols (flavonoids and phenolic acid), tocopherols, carotenoids, ascorbic acid, and essential oil constituents [181,182,183,184,185]