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2.9 Collection of the brain tissue samples Rats were euthanized through CO 2 asphyxiation within a glass chamber (Conlee et al., 2005)

[PubMed] National Institutes of Health

Outcomes The primary outcome was the measurement of total testosterone levels before and after GLP-1 RAs administration, chosen for its strong association with the clinical manifestations of hypogonadism in men

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Clinically, this mechanistic insight reinforces the importance of testing for mitochondrial stress markers and antioxidant status, particularly in individuals with a known or suspected toxicant burden