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Nuclear and Cytoplasmic Extract Preparation Nuclear and cytoplasmic extracts were prepared by using NE-PER nuclear and cytoplasmic extraction Kit (Thermo Scientific, Rockford, IL, USA) according to the instructions specified by the manufacturer

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Combination Rationale Research has shown that combining amylin analogues with GLP-1 receptor agonists produces synergistic weight loss effects that exceed either agent alone

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