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A considerable portion of the preclinical literature involves the administration of semaglutide prior to or concurrently with disease induction, thereby reflecting a prevention or very early intervention paradigm rather than a treatment approach

54, 55 Specifically, plasma samples were deproteinated by adding 50% v/v of freshly prepared 10% metaphosphoric acid (MPA), centrifuging at 2000 g for 2 min and then the supernatant stored at 20 C until assaying