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Some studies report increases in TMAO after supplementation in certain people, and the longterm cardiovascular significance of that finding remains an open research question.

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Endosomal pH investigation The effect of pH on FITC-agonist fluorescence was determined as fluorescence intensities [485 nm excitation, 520 nm emission (cut-off 495 nm)] of agonist solutions at specific pHs and data fitted using linear regression

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