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Statements Author contributions RM, XH wrote the manuscript

Safety depends on which peptides are combined and whether the protocol is supervised by a licensed provider

To demonstrate, let us return to Eliquis

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coli cells expressing GST fusion protein in pGEX vector Isopropyl-1-thio--D-galactopyranoside (IPTG), 100 mM stock 2.3 Affinity purification of GST fusion protein Glutathione Sepharose 4B bulk matrix (GE Healthcare or Sigma) (see Note 3) Column (BioRad 2.5 cm 10 cm Econo-Column) 10 mM glutathione buffer: 50 mM Tris, 10 mM reduced glutathione, pH 8.0 (make fresh daily) PBS/EDTA/PMSF: 1X PBS, 5 mM EDTA, 0.15 mM PMSF, pH 7.4 Pelleted E

Whooley, M