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Recombinant proteins in the supernatant were purified using nickelSepharose resin (GE Healthcare) and initially washed with wash buffer B1 (50 mM Tris, 500 mM NaCl and 30 mM imidazole, pH 8.0) until no protein was detected in the eluent using the Bradford reagent

Sharma, A., Sharma, A., Dixit, S

Therapies for hyperglycaemia-induced diabetic complications: from animal models to clinical trials

For small peptides this can help somewhat, but the data is thin

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SUGERENCIAS DE ENLACES EXTERNOS National Sleep Foundation - Sleep Science and Health: American Academy of Sleep Medicine - Sleep Medicine Research: NIH National Center on Sleep and Wakefulness: PubMed Central - Sleep Physiology Studies: European Sleep Research Society - Sleep Research: American Physiological Society - Sleep and Circadian Physiology: