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In the permutation test results of PLS-DA, Q2 was smaller than R2 and the value of the intersection of Q2 with the Y-axis was negative, proving that the model was applicable (Figure 3b)

Specifically, GRK5 upregulates HDAC5 expression and interacts with myocyte enhancer factor 2A (MEF2A) to suppress MEF2As transcriptional activity, thereby leading to reduced Smad7 transcription and enhanced Smad3 activation, ultimately promoting fibrotic progression

Methylation is involved in hundreds of biochemical reactions from DNA regulation to neurotransmitter synthesis to energy production

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Absorbance was recorded at 595 nm with a microplate reader (Tecan, Switzerland)