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Sections (4-mm thickness) were analyzed by immunohistochemistry using primary mouse monoclonal antibodies directed against CD4 (1 : 100 dilution), primary rat monoclonal antibody directed against F4/80 (1 : 100), and secondary biotinylated anti-mouse and anti-rat antibodies

This profile is further shaped by genetic predisposition, initial microbial exposure at birth, and long-term dietary habits (209)

The use of humanized mice was required, as it is well-established that human albumin binds poorly to mouse FcRn 13,70,71,72,73

For these five complications, the preferred disutility values (0.263, 0.280, 0.108, 0.164, and 0.090 for each complication, respectively) recommended by the systematic review were used in our base-case analysis, and the lowest value of each complication disutility value range (0.053, 0.177, 0.051, 0.007, and 0.027, respectively) was used in the sensitivity analyses