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204141) in a 50-l PCR reaction containing 1 l cDNA using an iCycler thermocycler (Bio-Rad Laboratories, Inc., Hercules, CA, USA) with the following thermocycling conditions: 50C for 2 min and 95C for 15 min, followed by 40 cycles of 94C for 15 sec, 55C for 30 sec and 72C for 30 sec

In summary, monitor the injection site (if pain increases or the area gets very red, hot, and swollen, inform your doctor as that could indicate injection-site infection, which is exceedingly rare with proper technique)

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The in vivo half-life study in hemizygous human FcRn Tg32 mice was performed at The Jackson Laboratory (JAX Services, Bar Harbor, ME, USA), which was approved by the Animal Care and Use Committee at The Jackson Laboratory