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The protein pellets were washed twice with prechilled methanol, resuspended in TEA buffer (5 mM Triethanolamine, 150 mM NaCl, 4% SDS, 4 mM EDTA, pH 7.4), and then incubated with 0.75 M of NH 2 OH for 1 h at room temperature to cleave palmitoylation thioester bonds