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Direct receptor expression in human coronary endothelial and smooth-muscle cells has been difficult to confirm using validated antibodies, raising the possibility that some vascular effects are mediated indirectly through circulating immune cells, hepatic signaling, or central-nervous-system pathways [14]

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The integrity of the obtained RNA was confirmed by the separation of the 28S and 18S rRNA bands in 2.0% agarose gel electrophoresis

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