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Indeed, a significantly lower glutathione-S-transferase (GST) enzyme activity and significantly high MDA levels have been shown in the cumulus cells of poor responders compared to high responders (220)

(5) Long-term effects: the long-term efficacy and safety of UA in treating neurodegenerative diseases remain uncertain, necessitating further long-term follow-up studies

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Cytochemical SA--galactosidase assay Cells were seeded in six-well plates, fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min, washed with 1 mM MgCl 2 /PBS (pH 6.0) and then incubated with X-Gal staining solution (1 mg ml 1 of X-Gal (Thermo Scientific, 2737087), 5 mM K 3 (Fe(CN) 6 ) and 5 mM K 4 (Fe(CN) 6 ) for 68 h at 37 C

J Agric Food Chem 55(8):28612866 Bones AM, Rossiter JT (2006) The enzymic and chemically induced decomposition of glucosinolates