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Selective and reversible modification of kinase cysteines with chlorofluoroacetamides

Anatomy, Bony Pelvis and Lower Limb, Foot Muscles

1 L cells were lysed by stirring in buffer containing 20 mM Tris-HCl, pH7.5, 0.2 g/ml leupeptin, 100 g/ml benzamidine, and 1 M PF-05190457 (GHSR antagonist) (Tocris)