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Blots were then washed and incubated with appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 h, and protein bands were visualized using a chemiluminescence kit (Thermo Scientific, Rockford, IL, USA)

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(Martyn Hooper et al, courtesy of the Pernicious Anaemia Society) Lord Prior of Bramptons response is word for word the same as the previous question, completely missing the point

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