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Digested samples were desalted using a C18 spin column (Pierce C18 Spin, #89873) according to manufacturer instructions prior to resuspension in EPPS buffer and CH 3 CN for TMT labeling as described above

The results showed a shift in carnitine metabolism that favored fat mobilization, meaning fat was more readily transported and burned for energy

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However, a recent study did not support this finding but rather attributed the increased risk of insulin resistance to the concomitant presence of metabolic dysfunctionassociated steatotic liver disease (MASLD) (81)