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Conclusion Understanding proper peptide storage and reconstitution techniques is essential for preserving their quality, stability, and structural integrity

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Beads were removed by centrifugation (500 g for 2 min), and the supernatant was collected and incubated with Ni-NTA resin to remove un-cleaved protein and remaining His tag

TM3 cells were harvested after treatment, stained with Annexin V-FITC and PI reagent, and incubated in the dark for 15 minutes