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Fluorescence images were acquired using a Zeiss LSM 900 fluorescence microscope, and myotube diameter was quantified using ImageJ

The gradient began at 1% B and ramped to 5% B in 0.1 min, increased to 30% B in 12.1 min, increased to 40% in 0.7 min and finally increased to 99% B in 0.1 min and was held for 2 min to wash the column for a total run time of 15 min

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