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Bands was sequentially incubated with 10 mM DTT ammonium bicarbonate solution (for 30 min at 56 C), for protein denaturation, 55 mM IAA ammonium bicarbonate solution (for 20 min, at room temperature (RT), protected from light) for protein acetylation and with 100 mM ammonium bicarbonate/acetonitrile (AMBIC/ACN 1:1 v/v) (for 30 min at RT) for the destaining process

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These findings suggest that hepatic SREBP-2 significantly contributes to cholesterol synthesis, cholesterol uptake, and bile acid synthesis regulation, and that its inhibition by GLP-1RAs is essential for improving cholesterol metabolism in PA-treated HepG2 cells

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If you see any cloudiness, discoloration, or floating particles, something has gone wrong