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Am J Physiol Gastrointest Liver Physiol (2012) 303(7):G77585.10.1152/ajpgi.00155.2012 152 KawamataYFujiiRHosoyaMHaradaMYoshidaHMiwaMet alA G protein-coupled receptor responsive to bile acids

[3] Slley E, Kun S, Szijrt I A, et al

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Cells were then placed on ice, washed with cold HBSS, and incubated for 10 minutes in cold acetic acid + 150 mM NaCl buffer, pH 2.9, to strip surface ligand