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The program of qPCR reaction included 95 C for 10 min, 40 cycles of 95 C for 5 s and 60 C for 15 s

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Mass Spectrometry (MS): While HPLC verifies purity, Mass Spectrometry confirms the precise identity of the molecules by measuring their mass-to-charge ratio

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It plays a short-lived but critical role in initiating muscle repair by activating satellite cells and signaling the early stages of tissue regeneration

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