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The PCR positive clones were further confirmed by restriction digestion analysis of recombinant His6-AV-Ubiquitin-Semapeptide (nucleotide sequence)- pET24 plasmid vectors using Ndel (NEB) + Xhol (NEB) at 37 C

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Short-term storage at 4C may be acceptable per protocol

These dynamics strengthen hospital-run specialty pharmacies (which take work in-house to remain 340B-eligible) ( [15] )

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