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The resin was washed with 100 ml of buffer T containing 1 M KCl, 20 mM Imidazole, 1 mM ATP, and 5 mM MgCl 2 and then 25 ml of buffer T containing 300 mM KCl

doi: 10.1056/NEJMoa1600869 [DOI] [PMC free article] [PubMed] [Google Scholar] 14.Wiggins T, Antonowicz SS, Markar SR

doi: 10.1016/j.chest.2017.03.020 15 CorcoranSEONeillLAJ

Id asked my primary care physician (PCP) if I might be a good candidate and if it was really true that weight loss might make my arthritic knees better (I was terrified of the looming prospect of knee surgery!)

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