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The cells at the proper confluence were subsequently treated with BPC157 for the indicated times

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One milliliter of a PBS-based reaction mixture containing propidium iodide (20 g/mL), RNase (0.2 g/mL), and 0.1% Triton X-100 (Sigma) was added to the cell samples at a density of 1 10 6 cells/mL and incubated in the dark for 40 min before a DNA content analysis was conducted through flow cytometry

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In T2DM patients, SGLT2i treatment significantly reduced the risk of progression to DKD by 45% (151)