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Insert the needle at a 45 to 90 degree angle into a pinched fold of skin on the abdomen, upper arm, or thigh

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Intuitively, generation of ROS in this process must be timely and spatially controlled to prevent excessive damage to the DNA: a recent report, in fact, describes a new role for the originally discovered superoxide dismutase, SOD1, that is recruited to the nucleus in response to specific stimuli 142